snail1  (Cell Signaling Technology Inc)

Journal: Molecular Medicine

Article Title: Platelets induce epithelial to mesenchymal transition in renal proximal tubular epithelial cells through TGF-β signaling pathway

doi: 10.1186/s10020-025-01355-7

Figure Lengend Snippet: Ticagrelor treatment leads to a reduction in fibrosis, and this effect is associated with the inhibition of epithelial-mesenchymal transition (EMT) in mice with diabetic kidney disease (DKD). A Relative mRNA expression levels of Col1a1 , Acta2 (α-SMA), Snai2 (Slug ) , Cdh1 (E-cadherin), and Cdh2 (N-cadherin) in kidney tissues were assessed in the control group, DKD group, and DKD group treated with the platelet inhibitor Ticagrelor by RT-qPCR. B Representative images show SNAIL1 immunostaining in renal tissue sections from the same groups. The percentage of SNAIL1-positive cells was quantified from 10 non-overlapping fields per region. C Picrosirius Red (PSR) staining in renal tissue sections shows collagen deposition. Quantification shows the percentage of PSR-positive area across 10 non-overlapping fields. Scale bar, 50 μm. Data are presented as mean ± SEM. Statistical analysis was performed using One-way ANOVA for comparisons involving more than two groups, except for Snai2 (graph A) which was analyzed using the Kruskal-Wallis test. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001

Article Snippet: Samples were incubated overnight at 4 °C with the following primary antibodies: mouse monoclonal IgG2aκ anti-E-cadherin (1:1000, BD Biosciences, #610182), rabbit monoclonal IgG anti-SNAIL1 antibody (1:100, Cell Signaling, #3879), mouse monoclonal IgG2aκ anti-αSMA antibody (1:150, Dako, #M0851), and rabbit monoclonal IgG anti-p21 antibody (1:200, Abcam, #ab188224).

Techniques: Inhibition, Expressing, Control, Quantitative RT-PCR, Immunostaining, Staining

Journal: Molecular Medicine

Article Title: Platelets induce epithelial to mesenchymal transition in renal proximal tubular epithelial cells through TGF-β signaling pathway

doi: 10.1186/s10020-025-01355-7

Figure Lengend Snippet: Platelet depletion attenuates fibrosis and abolishes partial EMT in UUO mouse model. A Relative mRNA expression levels of Col1a1 , Col3a1 , Acta2 , Snai2 , Cdh1 , and Cdh2 in the renal tissues from UUO isotype control compared with platelet depletion groups, measured by RT-qPCR. B Representative images show SNAIL1 staining in renal tissue section from UUO isotype control and UUO platelet depleted mice. The graph shows the percentage of SNAIL1-positive staining was quantified in 10 non-overlapping fields. C Picrosirius Red (PSR) staining shows collagen deposition, stained area were quantified similarly. Scale bar, 50 μm. Data are presented as mean ± SEM. Statistical analysis was performed using the Student’s t -test for comparison between two groups. * P < 0.05, ** P < 0.01

Article Snippet: Samples were incubated overnight at 4 °C with the following primary antibodies: mouse monoclonal IgG2aκ anti-E-cadherin (1:1000, BD Biosciences, #610182), rabbit monoclonal IgG anti-SNAIL1 antibody (1:100, Cell Signaling, #3879), mouse monoclonal IgG2aκ anti-αSMA antibody (1:150, Dako, #M0851), and rabbit monoclonal IgG anti-p21 antibody (1:200, Abcam, #ab188224).

Techniques: Expressing, Control, Quantitative RT-PCR, Staining, Comparison

Journal: Molecular Medicine

Article Title: Platelets induce epithelial to mesenchymal transition in renal proximal tubular epithelial cells through TGF-β signaling pathway

doi: 10.1186/s10020-025-01355-7

Figure Lengend Snippet: Platelets induce EMT in HK-2 cells. A This figure illustrates the in vitro experimental setup using renal HK-2 cells treated with either resting or activated platelets for 10 days. B The expression of epithelial and mesenchymal markers, as well as ( C ) EMT-related transcription factors, were quantified in HK-2 cell lysates using RT-qPCR. D Protein expressions were analyzed by immunoblotting and are presented as graphical results. E Morphological changes were analyzed by light microscopy in control HK-2 cells, resting platelet-treated cells, and activated platelet-treated cells. Representative immunofluorescence images show α-SMA, E-cadherin and SNAIL1 (green). Hoechst staining (blue) was used for nuclear visualization. Scale bars: 50 μm (α-SMA and SNAIL1) and 75 μm (E-cadherin). Data are presented as mean ± SEM. Statistical analysis was performed using One-way ANOVA for comparisons involving more than 2 groups, except for Vim (graph B) and ZEB1 (graph D), which were analyzed using the Kruskal-Wallis test. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001

Article Snippet: Samples were incubated overnight at 4 °C with the following primary antibodies: mouse monoclonal IgG2aκ anti-E-cadherin (1:1000, BD Biosciences, #610182), rabbit monoclonal IgG anti-SNAIL1 antibody (1:100, Cell Signaling, #3879), mouse monoclonal IgG2aκ anti-αSMA antibody (1:150, Dako, #M0851), and rabbit monoclonal IgG anti-p21 antibody (1:200, Abcam, #ab188224).

Techniques: In Vitro, Expressing, Quantitative RT-PCR, Western Blot, Light Microscopy, Control, Immunofluorescence, Staining